PROMEC Core

Overview of Services

 

Proteomics and Metabolomics Core Facility (PROMEC) offers service to support your research needs for proteomics and metabolomics. We kan provide a wide range of services, from protein and peptide separation, (1D/2D)-PAGE, 1D/2D-western, capillary western analysis and offgel isoelectric focusing, to mass-spectrometry-based protein identification, de-novo sequencing and analysis of post translational modifications. In the initial phase access to LC-MS instrumentation for metabolite analysis will be offered to trained users, aiming to gradually increase our capacity to offer such analyses as a service.

This core facility belongs to Department of Cancer Research and Molecular Medicine at Faculty of Medicine. PROMEC started as a node of the Norwegian Proteomics consortium NorProteomics, with the responsibility of doing proteomics analysis at NTNU/HMN and for others in the area of Mid-Norway. Our goal has been and will be to provide modern proteomics technology to Norwegian scientists with the best possible services and lowest cost.

 

We provide access to:

  • ABSciex 5000 triple-quadrupole in-line Schimazdu HPLC
  • ABSciex 5500 triple-quadrupole in-line Schimazdu HPLC
  • Thermo Orbitrap Elite in-line Thermo EASY nLC 1000 UPLC
  • Thermo Q Exactive in-line Thermo EASY nLC 1000 UPLC
  • 2D-PAGE (IGPhor, Ettan)
  • OffGel Fractionator
  • SIMON Gel free western
  • Ettan Spotpicker
  • Typhoon Trio Laser scanner (f. eks til DIGE-analyser etter Cy 2/3/5-labelling)

 

I addition we have access to other equipment in the closest lab for complementary protein analysis;laser-confocal microscopy, X-ray diffractometer (Oxford), chromatography equipment for protein separation (Akta FPLC, Akta Explorer), Western gel documentation (Kodak, Licor), beta-scintillation counter, centrifuges etc.

 

Personnel

 

Scientific Leader

Professor Geir Slupphaug

Phone: +47 72573076

Mobile phone: +47 91825455

E-mail: geir.slupphaug@ntnu.no

 

General Manager

Davi de M. Fonseca

Phone: +47 72573342

Mobile phone: +47 95031752

E-mail: davi.fonseca@ntnu.no

 

Technical Staff

 

Location and hours of operation

Hours Location
PROMEC is open during normal working
hours (08:00-16:00). Approved users may use the instruments 24 h a day all week

Laboratory Centre, 4th floor (east).
Room 231.04.K02 (right outside elevator). Two additional MS instruments in "Bevegelsessenteret"

Links and Resources

Contacts

Name Role Phone Email Location
Geir Slupphaug
Scientific Leader
 
+47 91825455
 
geir.slupphaug@ntnu.no
 
Laboratory Centre 5th floor East
 
Davi de M. Fonseca
General Manager
 
+47 95031752
 
davi.fonseca@ntnu.no
 
Laboratory Centre 4th floor East